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Coelentrazine-h (Yeasen Biotech) was added to a final concentration of 5 M for 5 min before bioluminescence resonance energy transfer (BRET) readings were made using an EnVision plate reader (PerkinElmer)

Subcellular fractionation revealed reductions in the cytosolic levels of G1 and arrestin3, and corresponding increases in G1 nuclear levels (Fig

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