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However, GLP-1 therapy is not recommended for those who are pregnant, breastfeeding, or have a family history of certain medical conditions

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PCR mixtures were in both cases prepared with 0.2 mM dNTPs, 1x Phusion GC buffer, 0.2 M of overlapping primers containing the desired mutation, 3% DMSO, and Phusion high-fidelity DNA polymerase