Specifically, nutrient absorption impairment caused by microplastic physical blockage of the digestive system likely contributed to these effects ( Physa fontinalis , amphipods, and rotifers also indicated reduced growth when exposed to microplastics ( Although we did not directly assess chemical interactions (i.e., synergistic, additive, antagonistic), the reduced growth observed with ibuprofen and microplastics combined may suggest that in addition to an impaired digestive system, oxidative stress from ibuprofen likely contributed to the effects of microplastics alone ( Chironomus riparius after ibuprofen exposure ( Danio rerio (zebrafish), ibuprofen increased oxidative stress markers, including catalase, glutathione S-transferase, and protein carbonyls ( Dreissena polymorpha (zebra mussel), ibuprofen elicited lipid peroxidation and disrupted enzymatic responses ( Reduced growth in P
coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)
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