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The gRNAs targeting a further nine genes, Tp63 , Ppm1d , Cul3 , Pik3ca , Ccnd1 , Zfp36l2 , Arid2 , Kmt2d and Nfe2l2 were depleted, indicating that loss of gene function resulted in negative selection

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Images were analyzed using the Adiposoft plug-in software in ImageJ (NIH).[22] Briefly, images were converted to 8-bit images and scald to 0.366 microns per pixel (corresponding to 20 magnification on the Leica microscope)