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Sigma-Aldrich) on ice for 30 min, then at 37C for 5 min, rinsed in 2% BSA in PBS, and dissociated with gentle pipetting

However, without controlled studies it remains unclear whether the degree of weight loss, the consistency of results, or the side effect profile matches what has been observed in clinical trials of FDA approved products

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I was hoping SOME of my appetite would return (especially since I was just on the higher dose) AND I didnt want hot flashes on vacay