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Cells were then resuspended with stimulation buffer (Hanks balanced salt solution (HBSS) supplemented with 5 mM HEPES, 0.5 mM IBMX, and 0.1% (w/v) BSA, pH 7.4) to a density of 0.6 million cells per mL and added to 384-well white plates (3,000 cells per well)

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It's really hard to lose weight, and a lot of people might need a medication or surgery or another medical intervention because obesity is a disease, Jay adds