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Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)

Lentivirus was used to transfect pancreatic cancer cells and downregulate c-Met and mTOR expression

Tunica adventitia The tunica adventitia consists primarily of immune cells, fibroblasts, a collagen-rich ECM, and connective tissue with the vasa vasorum, lymphatic system, and nerve plexus

Poston L, Igosheva N, Mistry HD, et al