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Were not just riding the Protein Soda wave

If nausea or reduced appetite develops after escalation, return to the previous tolerated dose and discuss next steps with your clinician

The cultivation was performed in 500 mL baffled shake flasks with 50 mL LB medium supplemented with antibiotics as stated above on a shaking incubator (37C, shaking frequency: 180 min 1 , eccentricity: 25 mm) with a starting OD 600 of 0.1