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Each method has advantages and disadvantages, and scientific studies have considered both

doi:10.1080/01443615.2016.1264375 Salehpour S, Nazari L, Hoseini S, Moghaddam PB, Gachkar L

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Therefore, a multiple standard addition approach was employed for method validation, enabling accurate quantification despite significant matrix effects without requiring isotope-labeled IS

16 Most studies included patients with various aetiologies of B12 deficiency, including pernicious anaemia, and improvements were seen across different causes