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The variability seen at the N-terminus of the Gcg protein sequences is not suggested to impact secretion as all precursor sequences were predicted to have signal peptides and do not have potentially Deleterious or Affect function substitutions ( Supplementary Figure 5 and Supplementary Table 8 )

11 Considering GIP analogues, Gly 2 GIP shows improved cAMP activity and insulinotropic activity in vitro, 20 while the cAMP activity of D-Ala 2 GIP matches that of GIP

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Conclusion Artificial intelligence holds substantial promise in transforming SCD care through improved prediction, personalization, and equity-driven resource allocation