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Together these peptides enable investigation of how intracellular structural regulation interfaces with signalling pathways governing cellular response coordination

In addition, mtDNA variations are common for those with ASD

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Protein Extraction and Immunoblot Analysis To analyze the levels of specific mitochondrial proteins in the muscle, 2 mg of lyophilised muscle was homogenized in ice-cold buffer (100 lmg 1 dry weight) consisting of 2 mM HEPES (pH 7.4), 1 mM EDTA, 5 mM EGTA, 10 mM MgCl 2 , 50 mM -glycerophosphate, 1% TritonX-100, 2 mM dithiothreitol, 1% phosphatase inhibitor cocktail (Sigma P-2850) and 1% (v/v) Halt Protease Inhibitor Cocktail (Thermo Scientific, Rockford, United States) using a Bullet Blender (NextAdvance, New York, United States)