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Samples were processed on ice and then were centrifuged at 13,200 g for 10 min at 4 C and 1X protease inhibitor in a microcentrifuge

References Carlsson-Skwirut, C., Lake, M., Hartmanis, M., Hall, K., & Sara, V

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Activation of the Nrf2-ARE signaling pathway by I3C and DIM leads to increased expression of cytoprotective genes, reducing inflammation and oxidative damage [151]