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To assess in vivo the response to SCFAs, GLP-1 levels were measured in WT and FXR KO mice supplemented with prebiotics (inulin-type fructans) to increase SCFA production in the colon

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c , The HEK293 cells (WT or PIGK deficient) expressing GFP-GPI and PIGK (WT or mutants) are analyzed according to GFP and Cy5 fluorescence using flow cytometry