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After centrifugation, protein extracts were collected and 50 g of protein lysates were resolved by SDS-PAGE and electrophoretically transferred onto Immobilon-P membranes (0.22 M

Finally, blastocysts from each group were collected and incubated with 5 g/ml Hoechst 33342 solution at 38C for 5 min, followed by blastocysts mounting and microscope examination with cell number per blastocyst ( n = 20 for each group) further recorded

[DOI] [PubMed] [Google Scholar] 31.Wang X., Song Z., Li H., Liu K., Sun Y., Liu X., Wang M., Yang Y., Su S., Li Z

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and oxygen saturation (SaO 2 ), 99% on room air