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Andrews, Duke University) were bred in-house at the University of Alberta Health Sciences Laboratory Animal Services facility

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Instead, you need to gently swirl the vial

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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles